Development and validation of new analytical method for the estimation of fluoxetine in bulk and dosage form by UV spectrophotometry

Authors

Uday sankar raju K Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Vimalakkannan T Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Ravindra Reddy K Department of Pharmaceutics, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Naveena P Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Riharika raj R Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Chinna subbarayudu S Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author
Hima bindu T Department of Pharmaceutical Analysis, P. Rami Reddy Memorial college of pharmacy, Kadapa, India. Author

Keywords:

Fluoxetine, UV Spectrophotometry

Abstract

A simple, rapid and precise method is developed for the quantitative determination of Fluoxetine in combined pharmaceutical-dosage forms. The method was based on UV Spectrophotometric determination of Fluoxetine drug using Beer-Lamberts Law. It involves absorbance measurement at 224 nm (λmax of Fluoxetine) in water. For UV Spectrophotometric method, linearity was obtained in concentration range of 5-30 mcg/ml with regression 0.999 for Fluoxetine respectively. Recovery was in the range of 98 -102%; the value of standard deviation and %R.S.D was found to be < 2 shows high precision of the method..