A Novel Extraction and Chromatographic Investigation of Phyto constituents in Sandal Seed

Abstract

One of the oldest and most precious sources of natural fragrance with significant Therapeutic and economic value is Santalum album L. (Santalaceae), sometimes referred to As Indian sandalwood. The cosmetics sector may make use of the oil that is derived from the seeds. Three families’ seed oils are the main source of natural ximenynic acid. Trans-11-octadecane-9-yonic acid is present in significant amounts in the seed oil of Santalum Album L. Following extraction, separation, and characterization using gas Chromatography, thin-layer chromatography, and high-performance liquid Chromatography techniques, the identity of xymenynic acid was verified. When Santalum Album L. seed oil was characterized and 12 fatty acids were identified, only the quantity of Ximenynic acid was discovered to be 66.76%. Nitrogen was used as the gas to help move Ximenynic acid, flowing at a rate of 1.37 milliliters per minute, after adjusting the testing Conditions. The ximenynic acid peak’s retention time was 17.743 minutes for the standard And 17.753 minutes for the sample. Chromatographic development was performed on a TLC plate precoated with silica gel 60 F254 using a mobile phase consisting of a 9:1 v/v Hexane: ethyl acetate combination. Xymenynic acid’s Rf value was determined to be 0.72 ± 0.01 .According to ICH criteria, this approach was verified and determined to be within the Limit. HPLC investigation was conducted using Phenomenex (C18, 250 x 4.6mm, 5 μ) Column was used to accomplish the chromatographic separation. UV detection at 229 nm was used .Temperature was maintained at 25°C using acetonitrile as a mobile phase for Ximenynic acid, with a flow rate of 1.0 mL/min. The ximenynic acid sample and standard were found to have retention times of 6.10 and 6.12 minutes, respectively.